by | Jan 15, 2020 | Wu, Gregory
— MOG.15 is a T cell hybridoma that will be active when engaged with an antigen presenting cell that is presenting MOG35-55 peptide.
Publication: Conditional MHC Class II Expression Reveals a Limited Role for B Cell Antigen Presentation in Primary and Secondary CD4 T Cell Responses
by | Jan 15, 2020 | Diwan, Abhinav
— Adenoviruses were generated using the Invitrogen Virapower system, which uses Clonase mediated recombination. They were generated in HEK293A cells and titered per the manufacturer’s instructions.
Publications: Enhancing lysosome biogenesis attenuates BNIP3-induced cardiomyocyte death; Impaired …
by | Jan 15, 2020 | Lowe, Mark, Xiao, Xunjun
— These plasmids encode carboxyl ester lipase (CEL) with and without genetic variants that are associated with chronic pancreatitis.
To make the plasmids, the researchers used a full-length human CEL cDNA clone. The cDNA encoded for a 14-repeat VNTR, the variant present in the CEL MODY family. The Ec…
by | Dec 18, 2019 | Solnica-Krezel, Lilianna
— Mutant zebrafish animals harboring chemically induced point mutations in the genome.
Animals: spon1b fh440, kiss1 stl82, kiss1rb fh438, pon2 fh450
by | Dec 18, 2019 | Diamond, Michael, Fernandez, Estefania, Fremont, Daved, Nelson, Christopher, Platt, Derek, Zhao, Haiyan
— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…