MOG.15 hybridoma

— MOG.15 is a T cell hybridoma that will be active when engaged with an antigen presenting cell that is presenting MOG35-55 peptide.

Publication: Conditional MHC Class II Expression Reveals a Limited Role for B Cell Antigen Presentation in Primary and Secondary CD4 T Cell Responses

Adenovirus: Ad-GFP-LC3, Ad-FLAG-BNIP3, Ad-mBECLIN-1, Ad-Parkin(HA), Ad-TRAF2(FLAG), Ad-TRAF2-Rm, Ad- TRAF2-deltaN. Plasmids: mCherry-GFP-LC3, DsRed-BNIP3, GFP-Parkin, GFP-LC3, DsRed-monomer-GFP fusion positive control

— Adenoviruses were generated using the Invitrogen Virapower system, which uses Clonase mediated recombination. They were generated in HEK293A cells and titered per the manufacturer’s instructions.

Publications: Enhancing lysosome biogenesis attenuates BNIP3-induced cardiomyocyte death; Impaired …

Plasmids with genetic variants of CEL

— These plasmids encode carboxyl ester lipase (CEL) with and without genetic variants that are associated with chronic pancreatitis.

To make the plasmids, the researchers used a full-length human CEL cDNA clone. The cDNA encoded for a 14-repeat VNTR, the variant present in the CEL MODY family. The Ec…

Monoclonal Antibodies Against Zika Virus and Purified Zika E Protein

— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…

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