— Materials that have been used to study transcription factor EB (TFEB), a lysosomal biogenesis factor:
Adenovirus: Ad-GFP-LC3, Ad-FLAG-BNIP3, Ad-mBECLIN-1, Ad-Parkin(HA), Ad-TRAF2(FLAG), Ad-TRAF2-Rm, Ad- TRAF2-deltaN. Plasmids: mCherry-GFP-LC3, DsRed-BNIP3, GFP-Parkin, GFP-LC3, DsRed-monomer-GFP fusion positive control
— Adenoviruses were generated using the Invitrogen Virapower system, which uses Clonase mediated recombination. They were generated in HEK293A cells and titered per the manufacturer's instructions.
Publications: Enhancing lysosome biogenesis attenuates BNIP3-induced cardiomyocyte death; Impaired …