Wfs1 gene knockout NSC34 cells
— Neuronal cell line lacking Wfs1 gene by CRISPR/CAS9
Toxoplasma strains
— The inventors have generated multiple gene knockout strains of T. gondii, listed below.
RH∆hx∆ku80 TIR1 (RH∆ku80∆hxgprt/pTub 5’UTR-OsTir1-3xFlag-DHFR 3’UTR-CAT):
The inventors used the CRISPR/Cas9 system for genome editing, comined with the AID degradation system to generate th…
HFF Cell Line
— This human cell line is a generic source for culturing Toxoplasma gondii in the laboratory. It was generated by Michael White’s laboratory.
Niemann-Pick C1 P1007A mouse model
— Mouse model for Niemann-Pick C1 (NPC1) disease
This mouse model was generated by CRISPR KI of the P1007A mutation into the NPC1 protein. Mice heterozygous or homozygous for this mutation do not have an apparent phenotype. However, when the heterozgous P1007A mouse is crossed with the NPC1 I1061T m…
Plasmids for Cryptosporidium parvum
— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum
To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …
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