Wfs1 gene knockout NSC34 cells
— Neuronal cell line lacking Wfs1 gene by CRISPR/CAS9
iPSC reporter BJFF6 cell lines
— These induced pluripotent stem cell reporter cell lines were generated by introducing the following fluorescent reporters at the indicated loci:
BJFF6 cells were modified with CRISPR/Cas9 to generate these cell lines.
HFF Cell Line
— This human cell line is a generic source for culturing Toxoplasma gondii in the laboratory. It was generated by Michael White’s laboratory.
Niemann-Pick C1 P1007A mouse model
— Mouse model for Niemann-Pick C1 (NPC1) disease
This mouse model was generated by CRISPR KI of the P1007A mutation into the NPC1 protein. Mice heterozygous or homozygous for this mutation do not have an apparent phenotype. However, when the heterozgous P1007A mouse is crossed with the NPC1 I1061T m…
Plasmids for Cryptosporidium parvum
— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum
To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …
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