pTrc-eYFP Construct

by | Dec 5, 2019 | Pakrasi, Himadri

— This self-replicating plasmid is a derivative of pPMQAK1-BBa_P1010 and contains an expression-enhanced YFP gene under the control of an inducible Trc promoter. This plasmid was used to demonstrate that transgenic DNA could be introduced to Synechococcus UTEX 2973 cyanobacteria cells using conjugatio…

Hybridoma Cell Lines SAG1 and MIC2

by | Nov 27, 2019 | Sibley, Laurence David

— 2 murine hybridoma cell lines producing antibodies agains Toxoplasma gondii proteins SAG1 and MIC2

SAG1 (DG52) publication: Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii

MIC2 (6D10) publication: The Toxoplasma Adhesive Protein MIC2 Is Proteolytically Proces…

Monoclonal Antibodies to Enterococcus Faecalis Secreted Proteins

by | Nov 27, 2019 | Shaikh, Nurmohammad, Tarr, Phillip

— Target: SalB (mAb 6H3)

Notes: Reduces inflammatory response.

We have identified and characterized monoclonal antibodies that recognize secreted proteins of Enterococcus faecalis, and that neutralize the abilities of whole, viable, E. faecilis to stimulate a pro-inflammatory cytokine in cultured epithelial cells. The monoclonal antibodies are directed against …