by | Nov 21, 2019 | Funkhouser-Jones, Lisa, Shen, Bang, Sibley, Laurence
— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum
To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …
by | Nov 13, 2019 | Funkhouser-Jones, Lisa, Sibley, Laurence
— Plasmids for luciferase expression in Cryptosporidium
The plasmids listed below are for targeting insertion into the TK and UPRT loci in Cryptosporidium parvum. To generate the plasmids, the inventors modified a previously described nanoluciferase (Nluc) reporter fused to neomycin resistance (NeoR)…
