Plasmids for Cryptosporidium parvum

by | Nov 21, 2019 | Funkhouser-Jones, Lisa, Shen, Bang, Sibley, Laurence

— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum

To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …