by | Dec 18, 2019 | Murphy, Kenneth
— Researchers first identified and then used CRISPR to delete an enhancer located at +32 kb of the Irf8 transcriptional start site in mice. Knocking out this enhancer revealed that interferon regulatory factor 8 (IRF8) is required for the development of classical type 1 dendritic cells (cDC1s) but not…
by | Nov 27, 2019 | Murphy, Kenneth
— Irf8 Delta32 mice have a CRISPR targetted deletion in the enhancer of the mouse Irf8 gene located at +32 kilobases from the Irf8 gene promoter. This deletion removes the binding site for the Jun/Batf3/Irf8 complex and results in a complete and permanent elimination of the development of the cDC1 lin…
by | Nov 13, 2019 | Durai, Vivek, Murphy, Kenneth, Murphy, Theresa
— Xcr1 IRES mCherry-hCre Mouse Strain
This genetically modified mouse strain expresses the fluorescent marker mCherry and a human codon optimzed Cre in the same transcript as the chemokine receptor XCR1. XCR1 is specifically expressed in cDC1s, allowing specific deletion of genes in and the fluoresce…
by | Nov 6, 2019 | Hurchla, Michelle, Murphy, Kenneth, Murphy, Theresa, Sedy, John
— Target: B and T lymphocyte attenuator (BTLA). BTLA is expressed on peripheral lymphocytes, splenic macrophages and select developing B and T cells.
Inventors created the monoclonal antibodies by immunizing Armenian hamster or BALB/c background BTLA -/- mice. Hamster monoclonal 6A6 is of the IgG isotype, whereas all murine Abs are IgG1κ. Some of the antibodies recognize the mouse BTLA C57/BL6 allele (6A6, 3F9.C6) and some are panspecific (…
by | Oct 22, 2019 | Murphy, Kenneth, Murphy, Theresa
— Notes: Btla-/- knockout mouse with a 129S/SvEv background. B6.129.Btla tm1kmm/J Mouse.
by | Oct 22, 2019 | Diamond, Michael, Murphy, Kenneth, Murphy, Theresa, Schreiber, Robert, Unanue, Emil
— Notes: Batf3-/- knockout mouse with a 129SvEv background.