— A plasmid containing the E. colil ColE1 origin of replication, a chloramphenicol (antibiotic) resistance cassette, and a tetracycline-inducible antisense RNA gene regulator containing a target binding region (TBR) and the synthetic MicF M7.4 Hfq binding site for additional stability in the RNA molec…
— Description:
Plasmids-
Publication:
DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS. Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630. ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
— A plasmid constuct containing the Streptococcus thermophilus dCas9 (dCas9sth 1) gene under the control of the arabinose inducible pBAD promoter and a constitutively expressed single guide RNA (sgRNA) on the pAL5000 vector backbone.
Publication: Molecular Toolkit for Gene Expression Control and Geno…
— The plasmid was constructed in E. coli DH10B which was grown in LB at 37°C and 250 rpm in 14 mL BD FalconTM round‐bottom tubes. Kanamycin (kan) (20 µg/mL) and spectinomycin (spec) (100 µg/mL) were added as appropriate. Plasmids were constructed using the…
— o pDD56: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.
o pDD57: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains a constitutively expressed GFP+ on…
— These set of plasmids allow for the repression and de-repression of fluorescent proteins (GFP and mCherry) through CRISPR interference and antisense RNA.
Publication: Programmable control of bacterial gene expression with the combined CRISPR and antisense RNA system
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Creating...
