by | Aug 13, 2020 | Hoynes-O'Connor, Allison, Moon, Tae Seok
— A plasmid containing the E. colil ColE1 origin of replication, a chloramphenicol (antibiotic) resistance cassette, and a tetracycline-inducible antisense RNA gene regulator containing a target binding region (TBR) and the synthetic MicF M7.4 Hfq binding site for additional stability in the RNA molec…
by | Nov 20, 2019 | Hoynes-O'Connor, Allison, Moon, Tae Seok
— These plasmids contain target binding regions (TBRs) targeting rfp that were placed under the control of pTet via blunt end lifgation. Hfq binding sites that had been identified from the literature were inserted adjacent to the TBR sequence using either blunt end ligation or Golden Gate assembly, de…
