The plasmid was constructed in E. coli DH10B which was grown in LB at 37°C and 250 rpm in 14 mL BD Falcon^TM round‐bottom tubes. Kanamycin (kan) (20 µg/mL) and spectinomycin (spec) (100 µg/mL) were added as appropriate. Plasmids were constructed using the Golden Gate assembly method (Engler et al., 2008) or blunt‐end ligation‐based cloning, and were sequence‐verified

Publication: Oxygen‐responsive genetic circuits constructed in Synechocystis sp. PCC 6803