by | Nov 20, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 85h protein, a.k.a. Ig-Like Transcripts 1.
Notes: Clone name is 24.
Wistar rats were immunized with ILT1/FLAG-transfected RBL cells. ILT1 mAb was selected by flow cytometry for staining ILT1/FLAG-transfected RBL cells. This antibody can be used to study myeloid cell activation.
Publication: Human Myeloid Cells Express an Activating ILT1 Receptor (ILT1) That Ass…
by | Nov 20, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 155 protein, a.k.a. the Polio Virus Receptor.
Notes: Clone available is 4.24. Used in flow cytometry.
To generate the antibodies, the researchers immunized rats with a recombinant protein consisting of the mCD155 extracellular region fused to human Fc and selected hybridomas that reacted with RMA-S–mCD155 transfectants but not with untransfected RMA-S cells.
Publication: DNAM-1 promotes activ…
by | Nov 20, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 226 protein, a.k.a. DNAX Accessory Molecule 1.
Notes: Clone available is 480.1
Inventors generated the antibodies by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL–mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293–mDNAM-1 or 293-mCD96 transfectants but not with untransfected 293 cells.
Publication: DNAM-1 pro…
by | Nov 20, 2019 | Colonna, Marco, Wilson, Timothy
— Target: Fc Receptor Like 4 protein.
Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.
Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…
by | Nov 14, 2019 | Colonna, Marco
— Target: Immunoglobulin transcript 3 (ILT3).
Notes: Clone name is ZM4.1.
To generate the hyrbidomas, 10-week-old, female BALB/c mice received an initial injection of ILT3 human IgG1 fusion protein mixed with Alu-Gel-S behind the neck. 4 weeks later, they were given a booster immunization with the same immunogen, followed after 2 weeks by a final injection of purified ILT…
by | Nov 14, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 96 protein, a.k.a. T Cell-Activated Increased Late Expression protein.
Notes: Clone name is MX92.39.
mAbs against CD96 (clone NK92.39, mouse IgG1) was obtained by immunizing mice with NK92 cells. This antibody can be used to study how NK cells recognize tumors.
Publication: Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by Interacting with the Poliovirus Receptor (CD155)
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