Office of Technology Management/Tech Transfer

WASHU INNOVATION CATALOG

Mouse Hybridoma producing IgG3 Antibodies against Human TIGIT (WUCAM)

by | Nov 27, 2019 | Boles, Kent, Colonna, Marco

— Target: T Cell Immunoreceptor with Ig and ITIM domains, a.k.a. WUCAM

Notes: Clone available is 4E1.2. Used in flow cytometry and functional studies.

Mouse IgG3 was generated by immunizing BALB/c mice with WUCAMFLAG-Baf3 cells. This can be used to study T cell-dependent antibody responses.

Publication: A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC

Rat Hybridoma producing IgG1 Monoclonal Antibodies to Mouse CD96

by | Nov 27, 2019 | Colonna, Marco

— Target: Cluster of Differentiation 96 protein.

Notes: Clone available is 3.3.1

We generated the mAb 480.1 anti – mouse DNAM-1 (mDNAM-1) or mAb 3.1 anti – mouse CD96 (mCD96) by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL – mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293 – mDNAM-1 or 293-mCD96 t…

Murine Hybridoma produing IgG2b Monoclonal Antibody to Human CRTAM – Cr24.1

by | Nov 21, 2019 | Colonna, Marco

— Target: Cluster of Differentation 355 protein, a.k.a. Cytotoxic and Regulatory T-Cell Molecule.

Notes: Clone available is Cr 24.1. Used in flow cytometry.

Monoclonal antibody to human CRTAM

Monoclonal antibody (mAb) Cr24.1 was generated by immunizing BALB/c mice with CRTAM-P815 cells. Cells were injected bilaterally 4 times at weekly intervals into the hind leg. Cytosine-phosphate-guanosine (CpG) oligonucleotide was used as adjuvant (CpG 1826, 25 &mu…

Mouse Hybridoma producing IgG2b Monoclonal Antibodies to Human FcRL4

by | Nov 20, 2019 | Colonna, Marco, Wilson, Timothy

— Target: Fc Receptor Like 4 protein.

Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.

Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…
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