Monoclonal antibody to human CRTAM
Monoclonal antibody (mAb) Cr24.1 was generated by immunizing BALB/c mice with CRTAM-P815 cells. Cells were injected bilaterally 4 times at weekly intervals into the hind leg. Cytosine-phosphate-guanosine (CpG) oligonucleotide was used as adjuvant (CpG 1826, 25 μg/injection). One day after the fourth injection, popliteal and inguinal lymph nodes were removed and processed to a single-cell suspension. Cells were fused to the SP2/0 myeloma. Positive hybridomas were selected by flow cytometry based on their supernatant's ability to stain CRTAM-P815 cells as opposed to mock-transfected P815 cells.