by | Nov 20, 2019 | Burgess, Robert, Sanes, Joshua
— This mouse strain has loxP sites flanking exons 3 and 4 of the ChAT gene. There is a Neo gene in the intron between exons 4 and 5. Mating these mice to A-Cre mice generates a null allele in subsequent generations, in which no ChAT protein or activity is detectable. The mice are maintained in the C57…
by | Nov 20, 2019 | Sanes, Joshua
— Myl-CFP Mice
The coding sequence for the enhanced CFP was inserted downstream of ~5kb of putative regulatory sequences from the mouse myosin Type I heavy chain gene. Transgenic mice were generated by pronuclear injection using standard methods.
JAX Stock No. 06922
MyIIA-RFP Mice
A ~100kb genomic…
by | Nov 20, 2019 | Schreiber, Robert
— Target: C-X-C chemokine family member (CXCR3). Expressed on NK cells, activated T lymphocytes, select
endothelial and epithelial cells.
Hamsters were immunized with a multiple antigens peptide-8 (MAPS-8) encompassing amino acids 1 to 37 of mouse CXCR3 in complete Freund’s adjuvant, and boosted with peptide in incomplete Freund’s adjuvant. Hamsters showing ELISA seropositivity for CXCR3 peptide were boosted and hybridomas gen…
by | Nov 13, 2019 | Listenberger, Laura, Ory, Daniel, Schaffer, Jean
— Gadd7 haploinsufficient mutant CHO cell line
Inventors generated a mutant Chinese Hamster Ovary (CHO) cell line with disruption of one allele encoding Gadd7, a non-coding RNA. CHO cells were transduced with retrovirus at a low multiplicity of infection and mutants were isolated. Number of retrovira…
by | Nov 13, 2019 | Schreiber, Robert
— Murine tumor cell lines
Murine syngeneic MCA induced tumor lines have been developed and characterized by the Schreiber Lab and provide critical models to study drugs or pathways that impact tumor growth or rejection. In particular they are used to study mechanism of action of immune checkpoint blo…