ANKO mice and ANKI mice
— Description:
Publications:
Kidney disease biomarkers for diagnosis, prognosis or clinical trials monitoring
— Technology Description
Researchers in Prof. Ying “Maggie” Chen’s laboratory have identified three sensitive, urine-based biomarkers of kidney disease that could be used for non-invasive, early detection and clinical trials monitoring. In particular, these mechanistic biomarkers a…
pBAD.dCas9sth1.pAL5000 plasmid
— A plasmid constuct containing the Streptococcus thermophilus dCas9 (dCas9sth 1) gene under the control of the arabinose inducible pBAD promoter and a constitutively expressed single guide RNA (sgRNA) on the pAL5000 vector backbone.
Publication: Molecular Toolkit for Gene Expression Control and Geno…
pTet-T – A plasmid for chromosomal integration of pTet-fbfp & kanamycin antibiotic resistance into Synechocystis sp. PCC6803
— The plasmid was constructed in E. coli DH10B which was grown in LB at 37°C and 250 rpm in 14 mL BD FalconTM round‐bottom tubes. Kanamycin (kan) (20 µg/mL) and spectinomycin (spec) (100 µg/mL) were added as appropriate. Plasmids were constructed using the…
Recombinant Alpha-synuclein monomer and fibrils produced from Recombinant Alpha-synuclein monomer
— Alpha synuclein is a 140 amino acid protein. The accumulation of aggregated alpha-synuclein within neuronal cells is the defining pathologic change in Parkinson’s disease. We produced recombinany human alpha-synuclein protein in E. Coli and purified it. We developmed an incubation procedure unde…
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