Cyanobacteria engineered for enhanced sucrose production

by | Sep 21, 2020 | Pakrasi, Himadri

— Cyanobacterial strains, including one with the highest productivity of sucrose in cyanobacteria to date.

Lin, P. C., Zhang, F., & Pakrasi, H. B. (2020). Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Scientific reports, 10(1), 1-8.

Suicide plasmid pSL2231

by | Jul 20, 2020 | Pakrasi, Himadri

— pSL2231 is a derivative of pBR322 that can be used to delete the nblA gene in Synechococcus UTEX 2973.

Publication: Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2

Shuttle vectors for gene expression in E. Coli and Cyanobacteria

by | Jul 20, 2020 | Pakrasi, Himadri

— Two shuttle vectors, pCA-UC118 and pCB-SC101, capable of replication in both Synechocystis 6803 and E. coli. Expression of reporter proteins were significantly enhanced in cells containing these new plasmids, thus providing superior gene expression platforms in this cyanobacterium.

Publication: …

Plasmids for cyanobacterial CRISPRi and high sucrose production

by | Jul 20, 2020 | Pakrasi, Himadri

— Plasmid for CRISPR interference in Synechococcus sp. UTEX 2973:

pSL3287 (Addgene), included in publication: Tunable repression of key photosynthetic processes using Cas12a CRISPR interference in the fast-growing cyanobacterium Synechoccoccus sp. UTEX 2973

Plasmids for high level of sucrose produ…

pTrc-eYFP Construct

by | Dec 5, 2019 | Pakrasi, Himadri

— This self-replicating plasmid is a derivative of pPMQAK1-BBa_P1010 and contains an expression-enhanced YFP gene under the control of an inducible Trc promoter. This plasmid was used to demonstrate that transgenic DNA could be introduced to Synechococcus UTEX 2973 cyanobacteria cells using conjugatio…