Plasmids for luciferase expression in Cryptosporidium

The plasmids listed below are for targeting insertion into the TK and UPRT loci in Cryptosporidium parvum. To generate the plasmids, the inventors modified a previously described nanoluciferase (Nluc) reporter fused to neomycin resistance (NeoR) by inserting a P2A skip peptide to increase luciferase expression and by adding GFP driven by the C. parvum actin promoter. The plasmids express either GFP or mCherry (mCH) as constitutive reporters.

Plasmid names:
TK-GFP-Nluc-P2A-neo-TK
UPRT-mCh-Nluc-P2A-neo-UPRT

Publication: A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability