by | Nov 20, 2019 | Sanes, Joshua
— Myl-CFP Mice
The coding sequence for the enhanced CFP was inserted downstream of ~5kb of putative regulatory sequences from the mouse myosin Type I heavy chain gene. Transgenic mice were generated by pronuclear injection using standard methods.
JAX Stock No. 06922
MyIIA-RFP Mice
A ~100kb genomic…
by | Nov 20, 2019 | Schreiber, Robert
— Target: C-X-C chemokine family member (CXCR3). Expressed on NK cells, activated T lymphocytes, select
endothelial and epithelial cells.
Hamsters were immunized with a multiple antigens peptide-8 (MAPS-8) encompassing amino acids 1 to 37 of mouse CXCR3 in complete Freund’s adjuvant, and boosted with peptide in incomplete Freund’s adjuvant. Hamsters showing ELISA seropositivity for CXCR3 peptide were boosted and hybridomas gen…
by | Nov 20, 2019 | Burgess, Robert, Sanes, Joshua
— This mouse strain has loxP sites flanking exons 3 and 4 of the ChAT gene. There is a Neo gene in the intron between exons 4 and 5. Mating these mice to A-Cre mice generates a null allele in subsequent generations, in which no ChAT protein or activity is detectable. The mice are maintained in the C57…
by | Nov 13, 2019 | Cooper, Megan
— This mouse strain has a genetic alteration in STAT3 and can be used to understand the role of STAT3 in human disease.
Publication: Early-onset lymphoproliferation and autoimmunity caused by germline STAT3 gain-of-function mutations
by | Nov 13, 2019 | Brookheart, Rita, Schaffer, Jean
— CHO Gadd7 knockdown cell lines
Inventors generated two stable Chinese Hamster Ovary (CHO) cell lines with knockdown of gadd7 by transfecting cells with a custom siRNA plasmid targeting hamster gadd7 (KD1 and KD2) and a control CHO cell line by transfecting cells with a custom scrambled siRNA plasmi…