by | Nov 20, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 226 protein, a.k.a. DNAX Accessory Molecule 1.
Notes: Clone available is 480.1
Inventors generated the antibodies by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL–mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293–mDNAM-1 or 293-mCD96 transfectants but not with untransfected 293 cells.
Publication: DNAM-1 pro…
by | Nov 20, 2019 | Colonna, Marco, Wilson, Timothy
— Target: Fc Receptor Like 4 protein.
Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.
Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…
by | Nov 20, 2019 | Colonna, Marco
— Target: Cluster of Differentiation 85h protein, a.k.a. Ig-Like Transcripts 1.
Notes: Clone name is 24.
Wistar rats were immunized with ILT1/FLAG-transfected RBL cells. ILT1 mAb was selected by flow cytometry for staining ILT1/FLAG-transfected RBL cells. This antibody can be used to study myeloid cell activation.
Publication: Human Myeloid Cells Express an Activating ILT1 Receptor (ILT1) That Ass…
by | Nov 20, 2019 | Ellebedy, Ali
— These are recombinant chimeric murine monoclonal antibodies that are directed against FimH, an adhesin within uropathogenic E coli (UPEC). The monoclonal antibodies were isolated from mice immunized with adjuvanted FimCH, but are expressed on a human IgG1 backbone and hence they are chimeric.
by | Nov 14, 2019 | Colonna, Marco
— Target: Immunoglobulin transcript 3 (ILT3).
Notes: Clone name is ZM4.1.
To generate the hyrbidomas, 10-week-old, female BALB/c mice received an initial injection of ILT3 human IgG1 fusion protein mixed with Alu-Gel-S behind the neck. 4 weeks later, they were given a booster immunization with the same immunogen, followed after 2 weeks by a final injection of purified ILT…
