Rat Hybridoma producing IgG Monoclonal Antibody to Human CD177 – Clone 4127
— Target: Cluster of Differentation 177 protein, a.k.a. Neutrophil Specific Antigen 1.
Notes: Clone available is 4127.
Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD155 (TAGE4)
— Target: Cluster of Differentiation 155 protein, a.k.a. the Polio Virus Receptor.
Notes: Clone available is 4.24. Used in flow cytometry.
To generate the antibodies, the researchers immunized rats with a recombinant protein consisting of the mCD155 extracellular region fused to human Fc and selected hybridomas that reacted with RMA-S–mCD155 transfectants but not with untransfected RMA-S cells.
Publication: DNAM-1 promotes activ…
Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD226 (DNAM-1)
— Target: Cluster of Differentiation 226 protein, a.k.a. DNAX Accessory Molecule 1.
Notes: Clone available is 480.1
Inventors generated the antibodies by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL–mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293–mDNAM-1 or 293-mCD96 transfectants but not with untransfected 293 cells.
Publication: DNAM-1 pro…
Mouse Hybridoma producing IgG2b Monoclonal Antibodies to Human FcRL4
— Target: Fc Receptor Like 4 protein.
Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.
Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…
Murine Hybridoma producing IgG2b Monoclonal Antibody to Human CD85h (ILT1)
— Target: Cluster of Differentiation 85h protein, a.k.a. Ig-Like Transcripts 1.
Notes: Clone name is 24.
Wistar rats were immunized with ILT1/FLAG-transfected RBL cells. ILT1 mAb was selected by flow cytometry for staining ILT1/FLAG-transfected RBL cells. This antibody can be used to study myeloid cell activation.
Publication: Human Myeloid Cells Express an Activating ILT1 Receptor (ILT1) That Ass…
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