by | Dec 11, 2019 | Miyoshi, Hiroyuki, Stappenbeck, Thaddeus
— Tangible Tool: Growth factor over-expressing cell line.
Notes: Produces conditioned media to grow various mammalian tissue stem cells.
These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine.
Publication: In vitro expansion and genetic…
by | Dec 5, 2019 | Andley, Usha
— Knock-in mice with a mutation in alpha-crystallin (cryaa-R49C) that causes cataracts. A knock-in plasmid was generated by cloning the 5′ and 3′ arms of the mouse RAcrystallin gene, followed by mutation and homologous recombination in mouse embryonic stem cells, which were implanted into …
by | Dec 5, 2019 | Dorn, Gerald
— Bnip3-KO mice: These were generated by replacing exons 2 and 3 with a neomycin resistance cassette. Recombinations were identified via southern blot.
Publication: Inhibition of ischemic cardiomyocyte apoptosis through targeted ablation of Bnip3 restrains postinfarction remodeling in mice
Nix KO m…
by | Dec 1, 2019 | Hawkins, William, Hotchkiss, Richard, Spitzer, Dirk
— WUSTL Technology 007646
Technology Description
Researchers at Washington University in St. Louis (WUSTL) have developed a novel TRAIL (TNF-related apoptosis inducing ligand)-based anticancer drug platform, designated TR3. TRAIL has potent and specific apoptotic activity against tumor cells. Thus,…
by | Dec 1, 2019 | Hawkins, William, Spitzer, Dirk
— WUSTL Technology 016481
Technology Description
Researchers at Washington University in St. Louis have developed an additional targeting strategy to provide more flexibility and improve the bioactivity of the anticancer therapeutic TR3. This strategy targets TR3 to mesothelin, a tumor biomarker fr…
