by | Jan 15, 2020 | Diwan, Abhinav
— Adenoviruses were generated using the Invitrogen Virapower system, which uses Clonase mediated recombination. They were generated in HEK293A cells and titered per the manufacturer’s instructions.
Publications: Enhancing lysosome biogenesis attenuates BNIP3-induced cardiomyocyte death; Impaired …
by | Dec 18, 2019 | Solnica-Krezel, Lilianna
— Mutant zebrafish animals harboring chemically induced point mutations in the genome.
Animals: spon1b fh440, kiss1 stl82, kiss1rb fh438, pon2 fh450
by | Dec 18, 2019 | Diamond, Michael, Fernandez, Estefania, Fremont, Daved, Nelson, Christopher, Platt, Derek, Zhao, Haiyan
— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…
by | Dec 11, 2019 | Miyoshi, Hiroyuki, Stappenbeck, Thaddeus
— Tangible Tool: Growth factor over-expressing cell line.
Notes: Produces conditioned media to grow various mammalian tissue stem cells.
These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine.
Publication: In vitro expansion and genetic…
by | Dec 5, 2019 | Andley, Usha
— Knock-in mice with a mutation in alpha-crystallin (cryaa-R49C) that causes cataracts. A knock-in plasmid was generated by cloning the 5′ and 3′ arms of the mouse RAcrystallin gene, followed by mutation and homologous recombination in mouse embryonic stem cells, which were implanted into …
