Fibroblast-Derived Cell Line Producing Wnt3a, R-spondin 3, & Noggin

— Tangible Tool: Growth factor over-expressing cell line.

Notes: Produces conditioned media to grow various mammalian tissue stem cells.

These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine.

Publication: In vitro expansion and genetic…

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