Split intein synthetic biology platform for scalable microbial production of high-performance biomimetic textiles, adhesives and other materials

— Brief Description

A team of engineers at Washington University in St. Louis have developed a synthetic biology platform for low-cost, sustainable production of high-performance composite materials. This technology uses a split intein assembly system to synthesize high molecular weight polymers (e.…

E. coli plasmids and strains for biofuel synthesis

— pA5c-tesA, pA8c-tesA-mleuABCD, pE2k-alsS-ilvCD

Researchers used a standard restriction digestion cloning based on the Biobrick platform or Gibson assembly to construct the plasmids. E. coli DH10B was used for cloning. These plasmids were designed to increase branch chain fatty acid production.

Pub…

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