E. coli plasmids and strains for biofuel synthesis
— pA5c-tesA, pA8c-tesA-mleuABCD, pE2k-alsS-ilvCD
Researchers used a standard restriction digestion cloning based on the Biobrick platform or Gibson assembly to construct the plasmids. E. coli DH10B was used for cloning. These plasmids were designed to increase branch chain fatty acid production.