Plasmids for cyanobacterial CRISPRi and high sucrose production

Tech ID: T-019345

Plasmid for CRISPR interference in Synechococcus sp. UTEX 2973:

pSL3287 (Addgene), included in publication: Tunable repression of key photosynthetic processes using Cas12a CRISPR interference in the fast-growing cyanobacterium Synechoccoccus sp. UTEX 2973

Plasmids for high level of sucrose production in Synechococcus sp. UTEX 2973:

pSL3005 – PlacUV5-cscB (E. coli), lacI, NS3-targeting [KanR]

(Addgene), included in publication: Enhance production of surcrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973

pSL3360 – Ptrc1O-sps (Synechocystis 6803), RSF1010 plasmid [GmR]

Included in publication: Enhance production of surcrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973

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Miller, Qian

qian.zhang@wustl.edu

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