— Adenoviruses were generated using the Invitrogen Virapower system, which uses Clonase mediated recombination. They were generated in HEK293A cells and titered per the manufacturer’s instructions. Publications: Enhancing lysosome biogenesis attenuates BNIP3-induced cardiomyocyte death; Impaired …
— The plasmid was constructed in E. coli DH10B which was grown in LB at 37°C and 250 rpm in 14 mL BD FalconTM round‐bottom tubes. Kanamycin (kan) (20 µg/mL) and spectinomycin (spec) (100 µg/mL) were added as appropriate. Plasmids were constructed using the…
— MOG.15 is a T cell hybridoma that will be active when engaged with an antigen presenting cell that is presenting MOG35-55 peptide. Publication: Conditional MHC Class II Expression Reveals a Limited Role for B Cell Antigen Presentation in Primary and Secondary CD4 T Cell Responses
— Cultured and sequenced bacterial strains isolated from fecal samples. The samples were collected from the Bill and Melinda Gates Foundation sponsored studies of childhood undernutrition in Bangladesh.
— Technology Description This technology is patented bipolar sieve electrode designed to stimulate peripheral nerves in a specific direction and thereby expand the potential of using this type of neural interface device in clinical applications such as nerve regeneration and prosthetics. Sieve elec…
— Technology Description A team of researchers at Washington University have developed ACALADE (Advanced Capability Lumbar Discectomy), a device designed to help surgeons safely access and quickly remove the back portions of herniated discs for faster, lower risk lumbar discectomy. Currently, about…
— Technology Description Researchers at Washington University in St. Louis have developed a method to chelate radium-223 and increase its utility in targeted alpha-particle therapy (TAT). TAT aims to deliver cytotoxic alpha-particles to cancer cells. Radium-223 is an alpha particle emitting isotope …
— Technology Description Researchers at Washington University in St. Louis have developed an electromyometrial imaging (EMMI) method to non-invasively monitor uterine contractions. During pregnancy, many women experience preterm contractions. Sometimes these contractions progress to pre-term labor a…
— A team of engineers from Washington University has developed a unique aerosol gel made from volume-spanning, semirigid networks of organic nanoparticles. Although the ultralow density and high surface area of these types of aerogels make them extremely useful for air and water purification, their pr…
— To generate a panel of antibodies against ZIKV, we serially infected Irf3/ mice 30 days apart with ZIKV MR-766 (Uganda, 1947) and ZIKV H/PF/2013 (French Polynesia, 2013). Irf3/ mice were used instead of wild-type (WT) mice, because ZIKV strains are deficient in evading type I interferon-mediated imm…
— Technology Description Engineers in Prof. Xuan “Silvia” Zhang’s laboratory have developed a unifying design and optimization paradigm to automate the design of analog-to-digital interfaces and create scalable, general purpose analog and mixed signal (AMS) blocks that employ machi…
— TZ4877 is a ligand for the sphingosine-1-phosphate (S1P) receptor 1 in the CNS, developed and radiolabeled with fluorine-18. TZ4877 is the standard (non-radioactive compound) which is used for HPLC analysis of the S1P1 PET tracer, [18F]TZ4877. TZ50153 is the radiolabeling precursor of the PET tracer…
— Using embryonic stem cells from the European knockout mouse consortium (EUCOMM), researchers developed transgenic mice with loxP sites flanking exon 3 of the mitochondrial pyruvate carrier 2 (Mpc2) gene. MPC2 is a component of the MPC complex, which is involved in the transport of pyruvate across th…
— U87 cells that express guassia luciferase under the control of the human LDLR promoter with constitutive HRP expression. WU878: PhLDLR/guassia luciferase WU988: pTL/guassia luciferase
— Researchers first identified and then used CRISPR to delete an enhancer located at +32 kb of the Irf8 transcriptional start site in mice. Knocking out this enhancer revealed that interferon regulatory factor 8 (IRF8) is required for the development of classical type 1 dendritic cells (cDC1s) but not…
— pA5c-tesA, pA8c-tesA-mleuABCD, pE2k-alsS-ilvCD Researchers used a standard restriction digestion cloning based on the Biobrick platform or Gibson assembly to construct the plasmids. E. coli DH10B was used for cloning. These plasmids were designed to increase branch chain fatty acid production. Pub…
— Using a series of Nf1 genetically-engineered mouse strains, researchers isolated low-grade glioma stem cells (CD133+ cells) for in vitro and in vivo brain tumor studies. These lines are authenticated optic glioma stem cells, with the ability to self-renew at limiting dilutions; express stem cell mar…
— This technology includes a plasmid with the full length ATP-binding cassette transporter A3 (ABCA3) cDNA cloned into the pAcGFP1-N1 vector and another plasmid with the ABCA3-GFP cDNA inserted into the pShuttle vector. Mutations in ABCA3 lead to severe neonatal respiratory distress syndrome and child…
— Researchers used CRISPR/Cas9-mediated gene targeting to knock in a GFP-P2A cassette into the ATG of the Six2 gene in human BJFF.6 induced pluripotent stem cells. The cells exhibit GFP expression in Six2+ cells when induced to differentiate towards the renal lineage. Six2 expression marks a progenito…
