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IRF8 +32 KO miceMurphy, Kenneth
T-018830
— Researchers first identified and then used CRISPR to delete an enhancer located at +32 kb of the Irf8 transcriptional start site in mice. Knocking out this enhancer revealed that interferon regulatory factor 8 (IRF8) is required for the development of classical type 1 dendritic cells (cDC1s) but not…
E. coli plasmids and strains for biofuel synthesisXiao, Yi ; Jiang, Wen ; Zhang, Fuzhong
T-017688
— pA5c-tesA, pA8c-tesA-mleuABCD, pE2k-alsS-ilvCD Researchers used a standard restriction digestion cloning based on the Biobrick platform or Gibson assembly to construct the plasmids. E. coli DH10B was used for cloning. These plasmids were designed to increase branch chain fatty acid production. Pub…
Nf1 Optic Glioma Stem CellsGutmann, David
T-017720
— Using a series of Nf1 genetically-engineered mouse strains, researchers isolated low-grade glioma stem cells (CD133+ cells) for in vitro and in vivo brain tumor studies. These lines are authenticated optic glioma stem cells, with the ability to self-renew at limiting dilutions; express stem cell mar…
ABCA3-GFP expression constructsWambach, Jennifer ; Cole, F. Sessions
T-017958
— This technology includes a plasmid with the full length ATP-binding cassette transporter A3 (ABCA3) cDNA cloned into the pAcGFP1-N1 vector and another plasmid with the ABCA3-GFP cDNA inserted into the pShuttle vector. Mutations in ABCA3 lead to severe neonatal respiratory distress syndrome and child…
Six2-GFP reporter iPSC line (subject to GE – GFP license)Humphreys, Benjamin
T-017760
— Researchers used CRISPR/Cas9-mediated gene targeting to knock in a GFP-P2A cassette into the ATG of the Six2 gene in human BJFF.6 induced pluripotent stem cells. The cells exhibit GFP expression in Six2+ cells when induced to differentiate towards the renal lineage. Six2 expression marks a progenito…
Mutant Zebrafish with chemically induced point mutationsSolnica-Krezel, Lilianna
T-017763
— Mutant zebrafish animals harboring chemically induced point mutations in the genome. Animals: spon1b fh440, kiss1 stl82, kiss1rb fh438, pon2 fh450
Automated, Improved Deformable Image Registration for Radiation Therapy and other Clinical ApplicationsYang, Deshan
T-017324
— Technology Description Prof. Deshan Yang and colleagues have developed automated systems and software toolkits to provide more accurate deformable image registration (DIR) for adaptive radiotherapy and other clinical applications. In particular, they have automated tedious and labor-intensive DIR …
Chimeric Artificial Interference RNA (aiRNA) for RNAi-based cancer therapyWang, Xiaowei
T-014021
— Technology Description In search of more effective cancer treatments, inventors in Dr. Xiaowei Wang’s lab at Washington University School of Medicine have developed artificial interference RNA (aiRNA). aiRNA is a patented chimeric RNAi that combines the multi-target abilities of miRNAs and t…
Armenian Hamster Anti-Mouse Tapasin Antibody, clone 5D3Wang, Xiaoli ; Lybarger, Lonnie ; Hansen, Ted
T-016628
— Target: Amino acids 11-34 of mouse tapasin. Application(s): Western Blotting Armenian hamster mAb (5D3) was produced to peptide EDAGGGGLSKKPATLLLRH GPRGP, representing residues 11–34 on mouse Tapasin (TPN) (44). TPN mAb was found to be specific for mouse TPN, as determined in a Western blot analysis of cell lysates of .221 (human TPN-positive) cells, .220 (human TPN-n…
St. Louis isolate of Bourbon virus (BRBV-STL)Boon, Adrianus "Jacco"
T-018815
— This strain of the Bourbon virus was isolated from the serum of an infected St. Louis-area patient. The isolated virus was used to demonstrate in vitro and in a mouse model that the antiviral drug favipiravir has prophylactic and therapeutic efficacy against the Bourbon virus, indicating it as a goo…
Mouse anti-MR1 hybridomas, clone 4E3 and 6D1Wang, Xiaoli ; Hansen, Ted
T-017622
— mAb 4E3 was derived by immunization of β2m-deficient BALB/c mice with a keyhole limpet hemocyanin-conjugated peptide corresponding to mMR1 residues 130–153 (L A M D Y V A H I T K Q A W E A N L H E L Q Y Q). Hybridomas were produced by standard methods and screened by anti-peptide ELISA. …
Armenian Hamster Anti-Mouse Properdin Monoclonal Antibody (clone H4)Bertram, Paula ; Hourcade, Dennis
T-014378
— Target: Full length mouse properdin Application(s): Western blot and immunofluorescenceTwo functional properties of one of the two hamster antibodies have been examined, the H4 subclone of the 3D12 clone. In experiments the H4 antibody was used to identify mouse properdin present in histological sections, to block mouse properdin function in vitro, and to ameliorate complement alterna…
Fibroblast-Derived Cell Line Producing Wnt3a, R-spondin 3, & NogginMiyoshi, Hiroyuki ; Stappenbeck, Thaddeus
T-014239
— Tangible Tool: Growth factor over-expressing cell line. Notes: Produces conditioned media to grow various mammalian tissue stem cells.These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine. Publication: In vitro expansion and genetic…
New PARP-1 Inhibitors for Imaging PARP-1 Expression in VivoRogers, Buck ; Xu, Jinbin ; Chu, Wenhua ; Zhou, Dong
T-018730
— [18F]WC-DZ-F is a derivative of [18F]FluorThanatrace and a radiotherapeutic agent. [18F]WC-DZ-F has suitable biodistribution profile, favourable logP and super metabolic stability in vivo. We have demonstrated that [18F]WC-DZ-F is capable of imaging PARP-1 expression in PC-3 prostate cancer xenograf…
SIMPEL: electrode device to safely and precisely stimulate sympathetic nerves to treat asthma, hypertension and other conditionsMacEwan, Matthew ; Moore, Amy ; Moran, Daniel ; Qin, Yidan (Ida) ; Ray, Wilson ; Zhang, Christopher ; Kamath, Ashwin ; Cloonan, Paige ; Leuthardt, Eric
T-017895
— Technology Description A team of researchers at Washington University have developed “SIMPEL”, a small, implanted electrode device designed to manage a variety of conditions (such as asthma, hypertension or neuropathy) by delivering current from a defined, fixed position near specific …
Mouse and Human Anti-MR1 HybridomaFremont, Daved ; Gilfillan, Susan ; Myers, Nancy ; Colonna, Marco ; Cella, Marina ; Hansen, Ted
T-013681
— Target: Major Histocompatibility Complex, Class 1-Related Protein Notes: Clone available is 26.5Hybridoma clone 26.5 secreting mAb 26.5 to human and mouse MR1, an MHC-like molecule that restricts the activation of anti-microbial MIAT cells. Publication: Evidence for MR1 Antigen Presentation to Mucosal-associated Invariant T Cells
Tumor-targeted fusion protein platform for enhanced cancer immunotherapyPachynski, Russell
T-015906
— Disease indication – cancer, particularly lung, breast, colon, and prostate cancer or melanoma (cancers where chemerin is down-regulated at the tumor site) Drug format – engineered fusion protein Drug class – first in class Research stage and Preliminary data Target &ndash…
Cryopreserved sperm from Bnip3-KO mice, Nix KO mice, and Nix floxed allele miceDorn, Gerald
T-018843
— Bnip3-KO mice: These were generated by replacing exons 2 and 3 with a neomycin resistance cassette. Recombinations were identified via southern blot. Publication: Inhibition of ischemic cardiomyocyte apoptosis through targeted ablation of Bnip3 restrains postinfarction remodeling in mice Nix KO m…
Plasmids for pDD56, pDD57, pDD87, pDD103, pDD108, pDD112, pDD120, pDD131, pDD132, and pDD143Moon, Tae Seok
T-018853
— o pDD56: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance. o pDD57: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains a constitutively expressed GFP+ on…
HoxB5 cDTR miceKarpova, Darja ; DiPersio, John
T-018897
— Transgenic HoxB5 conditional DTR (cDTR) mice have been generated using CRISPR mediated targeting of the HoxB5 gene (exon2) in fertilized wildtype C57Bl/6 eggs that were then microinjected into pseudopregnant wildtype female mice. The construct targeted under the controls of the endogenous HoxB5 prom…
