— mAb 4E3 was derived by immunization of β2m-deficient BALB/c mice with a keyhole limpet hemocyanin-conjugated peptide corresponding to mMR1 residues 130–153 (L A M D Y V A H I T K Q A W E A N L H E L Q Y Q). Hybridomas were produced by standard methods and screened by anti-peptide ELISA. …
— Target: Full length mouse properdin Application(s): Western blot and immunofluorescenceTwo functional properties of one of the two hamster antibodies have been examined, the H4 subclone of the 3D12 clone. In experiments the H4 antibody was used to identify mouse properdin present in histological sections, to block mouse properdin function in vitro, and to ameliorate complement alterna…
— Tangible Tool: Growth factor over-expressing cell line. Notes: Produces conditioned media to grow various mammalian tissue stem cells.These supportive L-WRN cells can produce conditioned media containing Wnt3a, R-spondin, and Noggin for the the support of gastrointestinal stem cells. This system can be used to make stem cell enriched epithelial spheroids from the mouse gastrointestine. Publication: In vitro expansion and genetic…
Mouse and Human Anti-MR1 Hybridoma Gilfillan, Susan ; Myers, Nancy ; Colonna, Marco ; Fremont, Daved ; Cella, Marina ; Hansen, Ted
T-013681
— Target: Major Histocompatibility Complex, Class 1-Related Protein Notes: Clone available is 26.5Hybridoma clone 26.5 secreting mAb 26.5 to human and mouse MR1, an MHC-like molecule that restricts the activation of anti-microbial MIAT cells. Publication: Evidence for MR1 Antigen Presentation to Mucosal-associated Invariant T Cells
— Disease indication – cancer, particularly lung, breast, colon, and prostate cancer or melanoma (cancers where chemerin is down-regulated at the tumor site) Drug format – engineered fusion protein Drug class – first in class Research stage and Preliminary data Target &ndash…
— Bnip3-KO mice: These were generated by replacing exons 2 and 3 with a neomycin resistance cassette. Recombinations were identified via southern blot. Publication: Inhibition of ischemic cardiomyocyte apoptosis through targeted ablation of Bnip3 restrains postinfarction remodeling in mice Nix KO m…
— o pDD56: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance. o pDD57: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains a constitutively expressed GFP+ on…
— Transgenic HoxB5 conditional DTR (cDTR) mice have been generated using CRISPR mediated targeting of the HoxB5 gene (exon2) in fertilized wildtype C57Bl/6 eggs that were then microinjected into pseudopregnant wildtype female mice. The construct targeted under the controls of the endogenous HoxB5 prom…
— Knock-in mice with a mutation in alpha-crystallin (cryaa-R49C) that causes cataracts. A knock-in plasmid was generated by cloning the 5′ and 3′ arms of the mouse RAcrystallin gene, followed by mutation and homologous recombination in mouse embryonic stem cells, which were implanted into …
— This self-replicating plasmid is a derivative of pPMQAK1-BBa_P1010 and contains an expression-enhanced YFP gene under the control of an inducible Trc promoter. This plasmid was used to demonstrate that transgenic DNA could be introduced to Synechococcus UTEX 2973 cyanobacteria cells using conjugatio…
— WUSTL Technology 007646 Technology Description Researchers at Washington University in St. Louis (WUSTL) have developed a novel TRAIL (TNF-related apoptosis inducing ligand)-based anticancer drug platform, designated TR3. TRAIL has potent and specific apoptotic activity against tumor cells. Thus,…
— WUSTL Technology 011885 Technology Description Researchers at Washington University in St. Louis have developed a strategy to specifically target the anticancer therapeutic TR3 to cancer cells using the mesothelin/MUC16 interaction. Mesothelin is a GPI linked cell surface protein expressed in mes…
— WUSTL Technology 016481 Technology Description Researchers at Washington University in St. Louis have developed an additional targeting strategy to provide more flexibility and improve the bioactivity of the anticancer therapeutic TR3. This strategy targets TR3 to mesothelin, a tumor biomarker fr…
— Technology Description Researchers at Washington University in St. Louis have developed a vaccine strategy to prevent catheter-associated urinary tract infections (CAUTIs). CAUTIs are the most common cause of hospital-acquired infections. Many of these are caused by Enterococcus bacteria. CAUTIs a…
— Technology Description Researchers from Washington University in St. Louis and colleagues have identified ent-allopregnanolone (ent-AlloP) as a potential therapeutic to treat glaucoma. Glaucoma, the leading cause of irreversible blindness, results from damage to the retinal ganglion cells (RGSs) t…
— Target: T Cell Immunoreceptor with Ig and ITIM domains, a.k.a. WUCAM Notes: Clone available is 4E1.2. Used in flow cytometry and functional studies.Mouse IgG3 was generated by immunizing BALB/c mice with WUCAMFLAG-Baf3 cells. This can be used to study T cell-dependent antibody responses. Publication: A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC
— Target: Cluster of Differentiation 96 protein. Notes: Clone available is 3.3.1We generated the mAb 480.1 anti – mouse DNAM-1 (mDNAM-1) or mAb 3.1 anti – mouse CD96 (mCD96) by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL – mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293 – mDNAM-1 or 293-mCD96 t…
— Target: SalB (mAb 6H3) Notes: Reduces inflammatory response.We have identified and characterized monoclonal antibodies that recognize secreted proteins of Enterococcus faecalis, and that neutralize the abilities of whole, viable, E. faecilis to stimulate a pro-inflammatory cytokine in cultured epithelial cells. The monoclonal antibodies are directed against …
— Plasmids were generated with the NHX-2 promoter, to restrict gene expression to the C. elegans intestinal cells, driving C. elegans genes or targeting peptides, known to target different subcellular organelles, fused to a C. elegans optimized mOrange2 fluorescent protein (CemOrange2). These expressi…
— 2 murine hybridoma cell lines producing antibodies agains Toxoplasma gondii proteins SAG1 and MIC2 SAG1 (DG52) publication: Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii MIC2 (6D10) publication: The Toxoplasma Adhesive Protein MIC2 Is Proteolytically Proces…
