ABCA3-GFP expression constructs

Tech ID: T-017958

This technology includes a plasmid with the full length ATP-binding cassette transporter A3 (ABCA3) cDNA cloned into the pAcGFP1-N1 vector and another plasmid with the ABCA3-GFP cDNA inserted into the pShuttle vector. Mutations in ABCA3 lead to severe neonatal respiratory distress syndrome and childhood interstitial lung disease. These plasmids were used in a study on the pathogenicity of two mutant ABCA3 alleles (R288K and R1474W), which found that both mutations encode proteins with reduced ATPase activity but normal intracellular localization and protein processing.

Publication: Functional Characterization of ATP-Binding Cassette Transporter A3 Mutations from Infants with Respiratory Distress Syndrome

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Hanway, Patrick

hanway@wustl.edu

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