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Ncr1-ERT2iCre Mice
Fehniger, Todd
T-018981

— This mouse strain is a novel Natural Killer cell specific inducible cre that is useful for studying the development, maturation, and function of NK cells. It has a tamoxifen inducible Ncr1-ERT2iCre mouse allele that is used for floxing gene(s) of interest out of NK cells. This model was designed, cr…

Target and small molecule drugs to selectively neutralize bacteria in urinary tract infections
Henderson, Jeffrey P.
T-018628

— Technology Description Researchers in Prof. Jeffrey Henderson’s laboratory have discovered a drug target and a class of small molecule candidates that could be used to selectively prevent pathogenic enterobacteria from causing diseases such as urinary tract infections (UTIs). Currently, UTI…

Murine Hybridoma producing IgG2b Monoclonal Antibody to Human CRTAM – Clone 134
Colonna, Marco
T-007609

— Target: Cluster of Differentation 355 protein, a.k.a. Cytotoxic and Regulatory T-Cell Molecule. Notes: Clone available is 134.

Rat Hybridoma producing IgG Monoclonal Antibody to Human CD177 – Clone 4127
Colonna, Marco
T-007606

— Target: Cluster of Differentation 177 protein, a.k.a. Neutrophil Specific Antigen 1. Notes: Clone available is 4127.

Murine Hybridoma produing IgG2b Monoclonal Antibody to Human CRTAM – Cr24.1
Colonna, Marco
T-007608

— Target: Cluster of Differentation 355 protein, a.k.a. Cytotoxic and Regulatory T-Cell Molecule. Notes: Clone available is Cr 24.1. Used in flow cytometry.Monoclonal antibody to human CRTAM Monoclonal antibody (mAb) Cr24.1 was generated by immunizing BALB/c mice with CRTAM-P815 cells. Cells were injected bilaterally 4 times at weekly intervals into the hind leg. Cytosine-phosphate-guanosine (CpG) oligonucleotide was used as adjuvant (CpG 1826, 25 …

Niemann-Pick C1 P1007A mouse model
Ory, Daniel
T-018918

— Mouse model for Niemann-Pick C1 (NPC1) disease This mouse model was generated by CRISPR KI of the P1007A mutation into the NPC1 protein. Mice heterozygous or homozygous for this mutation do not have an apparent phenotype. However, when the heterozgous P1007A mouse is crossed with the NPC1 I1061T m…

B2m Floxed mouse
Bern, Michael ; Yokoyama, Wayne
T-018951

— To generate the mice, the researchers micro-injected ES cell clones carrying the targeted allele B2mtm1a(EUCOMM)Hmgu (B2mtm1a) into Albino B6 blastocysts.Chimeric mice were bred to Albino B6 to identify germline transmission by coat color. Mice containing the germline-transmitted B2mtm1a allele were…

Plasmids for Cryptosporidium parvum
Shen, Bang ; Funkhouser-Jones, Lisa ; Sibley, Laurence
T-018967

— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …

HFF Cell Line
Sibley, Laurence
T-018969

— This human cell line is a generic source for culturing Toxoplasma gondii in the laboratory. It was generated by Michael White’s laboratory.

iPSC reporter BJFF6 cell lines
Jain, Sanjay
T-018986

— These induced pluripotent stem cell reporter cell lines were generated by introducing the following fluorescent reporters at the indicated loci: BJFF6 cells were modified with CRISPR/Cas9 to generate these cell lines.

Hamster Hybridoma producing Monoclonal Antibody against Mouse CXCR3
Schreiber, Robert
T-005883

— Target: C-X-C chemokine family member (CXCR3). Expressed on NK cells, activated T lymphocytes, select endothelial and epithelial cells.Hamsters were immunized with a multiple antigens peptide-8 (MAPS-8) encompassing amino acids 1 to 37 of mouse CXCR3 in complete Freund’s adjuvant, and boosted with peptide in incomplete Freund’s adjuvant. Hamsters showing ELISA seropositivity for CXCR3 peptide were boosted and hybridomas gen…

Murine Hybridoma producing IgG2b Monoclonal Antibody to Human CD85h (ILT1)
Colonna, Marco
T-005807

— Target: Cluster of Differentiation 85h protein, a.k.a. Ig-Like Transcripts 1. Notes: Clone name is 24.Wistar rats were immunized with ILT1/FLAG-transfected RBL cells. ILT1 mAb was selected by flow cytometry for staining ILT1/FLAG-transfected RBL cells. This antibody can be used to study myeloid cell activation. Publication: Human Myeloid Cells Express an Activating ILT1 Receptor (ILT1) That Ass…

Mouse Hybridoma producing IgG2b Monoclonal Antibodies to Human FcRL4
Wilson, Timothy ; Colonna, Marco
T-007612

— Target: Fc Receptor Like 4 protein. Notes: Clone available is 413D12. Used in flow cytometry, immunoprecipitation, and functional studies.Balb/c mice were immunized three times at two week intervals with paraformaldehyde-fixed FcRL4 stable transfectants, first in CFA, then twice in IFA + CpG 1826. Three days prior to fusion with SP2/0 cells, the final immunization was performed by IP injection of irradiated cells in PBS. Hybridoma clo…

Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD226 (DNAM-1)
Colonna, Marco
T-007610

— Target: Cluster of Differentiation 226 protein, a.k.a. DNAX Accessory Molecule 1. Notes: Clone available is 480.1Inventors generated the antibodies by immunizing rats with RBL cells transfected with mDNAM-1 cDNA (RBL–mDNAM-1) or mouse CD96 cDNA (RBL-mCD96) and selecting hybridomas that reacted with 293–mDNAM-1 or 293-mCD96 transfectants but not with untransfected 293 cells. Publication: DNAM-1 pro…

Rat Hybridoma producing IgG2a Monoclonal Antibodies to Murine CD155 (TAGE4)
Colonna, Marco
T-007611

— Target: Cluster of Differentiation 155 protein, a.k.a. the Polio Virus Receptor. Notes: Clone available is 4.24. Used in flow cytometry.To generate the antibodies, the researchers immunized rats with a recombinant protein consisting of the mCD155 extracellular region fused to human Fc and selected hybridomas that reacted with RMA-S–mCD155 transfectants but not with untransfected RMA-S cells. Publication: DNAM-1 promotes activ…

Hnf4a-Foxa1 fusion plasmid
Morris, Samantha
T-019160

— Plasmid with fused Hnf4a and Foxa1 genes to express as a single transcript. Individual genes were obtained from Addgene.

DNA plasmids to control gene expression in E. coli
Hoynes-O'Connor, Allison ; Moon, Tae Seok
T-019135

— These plasmids contain target binding regions (TBRs) targeting rfp that were placed under the control of pTet via blunt end lifgation. Hfq binding sites that had been identified from the literature were inserted adjacent to the TBR sequence using either blunt end ligation or Golden Gate assembly, de…

Transgenic mouse lines with markers of muscle development
Sanes, Joshua
T-019127

— Myl-CFP Mice The coding sequence for the enhanced CFP was inserted downstream of ~5kb of putative regulatory sequences from the mouse myosin Type I heavy chain gene. Transgenic mice were generated by pronuclear injection using standard methods. JAX Stock No. 06922 MyIIA-RFP Mice A ~100kb genomic…

Lyz2Cre;Talin-1fl/fl mice
Randolph, Gwendalyn
T-019085

— Lyz2Cre;Talin-1fl/fl mouse strain Inventors crossed Talin-1fl/fl mice (received from Oklahoma Medical Research Foundation) with Lyz2Cre mice (available at the Jackson Laboratory) to generate Lyz2Cre;Talin-1fl/fl mice.

Floxed ChAT mouse strain
Burgess, Robert ; Sanes, Joshua
T-019107

— This mouse strain has loxP sites flanking exons 3 and 4 of the ChAT gene. There is a Neo gene in the intron between exons 4 and 5. Mating these mice to A-Cre mice generates a null allele in subsequent generations, in which no ChAT protein or activity is detectable. The mice are maintained in the C57…