— Technology Description Researchers at Washington University in St. Louis have developed an improved method to monitor the progression of lymphoproliferative disorders (LPDs) and guide treatment decisions. LPDs, including leukemia and lymphoma, are characterized by the abnormal proliferation of blo…

— VP35 is involved in multiple functions crticial to viral replication in filoviruses such as Ebola and Marburg virus. Viruses with VP35 mutations are attenuated in in vivo models. 5.4 million compounds were screened for their ability to bind to VP35 at the key protein-to-protein interface. The screen…

— This mouse strain was designed to harbor a specific NF1 patient germline NF1 gene mutation.

— Using CRISPR-based gene editing, decorin was inserted downstream of Smad7 to create autoregulated stem cells that can produce decorin. As Smad7 is activated during TGF-beta signaling, the stems cells produce decorin in response to TGF-beta. Decorin is a key modulator of extracellular matrix assembly…

— Dr. Fumihiko Urano is director of the Wolfram Syndrome International Registry at WU and has developed many research tools for studying Wolfram Syndrome. The following constructs contain the wildtype WFS1 gene with various flags, or mutations of WFS that cause mild forms of Wolfram Syndrome (019395)…

— A mouse model of Wolfram Syndrome carrying a pathogenic variant of Wfs1 gene corresponding to human WFS1, c.1672C > T (p.R558c) missense mutation in exon 8 of WFS1.

— A humanized rat model of Wolfram Syndrome carrying a Wfs1 gene mutation corresponding to human WFS1 gene point mutation with a stop codon at position a.a. 452.

— Mouse with floxed elastin gene to conditionally inactivate elastin gene expression. Publication: Heterogeneous Cellular Contributions to Elastic Laminae Formation in Arterial Wall Development

— MCB6A organoids generate urothelial tumors with features similar to the basal-squamous subtype of urothelial carcinoma, the most common bladder cancer. MCB6A harbors 1,524 mutations, including mutations in Kdm6a and Trp53. Publication: CD4+ T cells induce rejection of urothelial tumors after imm…

— Plasmid for CRISPR interference in Synechococcus sp. UTEX 2973: pSL3287 (Addgene), included in publication: Tunable repression of key photosynthetic processes using Cas12a CRISPR interference in the fast-growing cyanobacterium Synechoccoccus sp. UTEX 2973 Plasmids for high level of sucrose produ…

— Two shuttle vectors, pCA-UC118 and pCB-SC101, capable of replication in both Synechocystis 6803 and E. coli. Expression of reporter proteins were significantly enhanced in cells containing these new plasmids, thus providing superior gene expression platforms in this cyanobacterium. Publication: …

— An UPAB1 derivative, UPAB1p-, that spontaneously loss pAB5 was identified by detection of restored Hcp secretion and isolated via an ELISA. Compared to wildype UPAB1, UPAB1p- displayed comparable growth in vitro in both rich medium and urine from healthy donors, indicating that less pAB5 did not in…

— pSL2231 is a derivative of pBR322 that can be used to delete the nblA gene in Synechococcus UTEX 2973. Publication: Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2

— Irg1−/− mice were generated after receiving embryonic stem cells (ESCs) from the Knockout Mouse Project Repository containing an insertion cassette between exons 3 and 4. This cassette prevents transcription of downstream exons 4 and 5 and production of mature protein. Irg1−/&minu…

— Technology Description Researchers at Washington University in St. Louis have developed new compounds that may be used to treat alcohol use disorder (AUD). AUD, a common condition that takes a high personal and public health toll, is characterized by severe problems with drinking- sufferers may co…

— Technology Description Researchers in Prof. Mario Feldman’s laboratory have developed a patented, versatile, cost-effective platform for creating new multivalent vaccines for a variety of Gram-negative and Gram-positive bacteria. This technology, glycoengineered outer membrane vesicles (geOM…

— Background: Recombinant vesicular stomatitis virus (VSV) has successfully been used to create a vaccine for Ebola virus that is currently on the market. Previously, Professor Sean Whelan and colleagues have exploited the VSV platform to create similar vectors for both SARS and MERS. Technology: The…

— Technology Description An interdisciplinary team of researchers has developed a photoacoustic near-infrared (PANIR) probe to predict preterm birth by quantifying water content in cervical tissue (an early and objective indicator of cervical remodeling). This compact photoacoustic endoscopy probe u…

— Technology Description An interdisciplinary team of researchers has developed a photoacoustic near-infrared (PANIR) probe to predict preterm birth by quantifying water content in cervical tissue (an early and objective indicator of cervical remodeling). This compact photoacoustic endoscopy probe u…

— Venezuelan equine encephalitis virus (VEEV) is an emerging infectious disease in South America transmitted by mosquitos. VEEV usually resides in forest and swamp areas where human populations are limited, however, VEEV has the potential to cause epidemics in humans and equines. LDLRAD3 has been foun…