— Target: SalB (mAb 6H3) Notes: Reduces inflammatory response.We have identified and characterized monoclonal antibodies that recognize secreted proteins of Enterococcus faecalis, and that neutralize the abilities of whole, viable, E. faecilis to stimulate a pro-inflammatory cytokine in cultured epithelial cells. The monoclonal antibodies are directed against …
— Technology Description Inventors in Washington University’s Department of Radiation Oncology have developed a method and 3D-printed applicator for intensity modulated high-dose-rate brachytherapy that surpasses conventional brachytherapy efficiency. This device is tailored to the patient …
— Plasmids were generated with the NHX-2 promoter, to restrict gene expression to the C. elegans intestinal cells, driving C. elegans genes or targeting peptides, known to target different subcellular organelles, fused to a C. elegans optimized mOrange2 fluorescent protein (CemOrange2). These expressi…
— 2 murine hybridoma cell lines producing antibodies agains Toxoplasma gondii proteins SAG1 and MIC2 SAG1 (DG52) publication: Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii MIC2 (6D10) publication: The Toxoplasma Adhesive Protein MIC2 Is Proteolytically Proces…
— The inventors have generated multiple gene knockout strains of T. gondii, listed below. RH∆hx∆ku80 TIR1 (RH∆ku80∆hxgprt/pTub 5’UTR-OsTir1-3xFlag-DHFR 3’UTR-CAT): The inventors used the CRISPR/Cas9 system for genome editing, comined with the AID degradation system to generate th…
— These set of plasmids allow for the repression and de-repression of fluorescent proteins (GFP and mCherry) through CRISPR interference and antisense RNA. Publication: Programmable control of bacterial gene expression with the combined CRISPR and antisense RNA system
— Irf8 Delta32 mice have a CRISPR targetted deletion in the enhancer of the mouse Irf8 gene located at +32 kilobases from the Irf8 gene promoter. This deletion removes the binding site for the Jun/Batf3/Irf8 complex and results in a complete and permanent elimination of the development of the cDC1 lin…
— CRISPR-Cas9 gene editing was used to modify the coding region of the SAMD9L mouse gene. The genetic modification resulted in the deletion of the full length production of the protein. This modification was made in a C57BL/6 genetic background.
— pRRLsinPGK-GFPppt : Plasmid DNA directing expression of green fluorescent protein in a lentiviral shuttle vector under the control of the phosphoglycerate kinase (PGK) promoter. FCIV: Plasmid DNA directing expression of Venus (a fluorescent protein) in a lentiviral shuttle vector under the control o…
— CRISPR-Cas9 gene editing was used to modify the coding region of the NMI mouse gene. The genetic modification resulted in the deletion of the full length production of the protein. This modification was made the C57BL/6J genetic background. NMI is a host protein involved in innate immune signaling a…
— CRISPR-Cas9 gene editing was used to modify the coding region of the MAP3K6 mouse gene. The genetic modification resulted in the deletion of the full length production of the protein. This modification was made in a C57BL/6J genetic background. MAP3K6 is a signaling molecule and could be the target …
— A plasmid based reverse genetics system for Bourbon virus was created. This system and related plasmids allow for the creation of infectious Bourbon virus and evaluate the impact of genetic variation on the virus (replication, disease, antibody-mediated neutralization etc.). It also allows for the c…
— This mouse strain is a novel Natural Killer cell specific inducible cre that is useful for studying the development, maturation, and function of NK cells. It has a tamoxifen inducible Ncr1-ERT2iCre mouse allele that is used for floxing gene(s) of interest out of NK cells. This model was designed, cr…
— Technology Description Researchers in Prof. Niraj Tolia’s laboratory have exploited structure-based vaccine design to develop immunogenic compositions and neutralizing antibodies that could protect against malaria and a range of other parasitic diseases that affect humans, livestock and comp…
— Technology Description Researchers in Prof. Jeffrey Henderson’s laboratory have discovered a drug target and a class of small molecule candidates that could be used to selectively prevent pathogenic enterobacteria from causing diseases such as urinary tract infections (UTIs). Currently, UTI…