CemOrange2 gene fusions for subcellular imaging in C. elegans

Tech ID: T-018982

Plasmids were generated with the NHX-2 promoter, to restrict gene expression to the C. elegans intestinal cells, driving C. elegans genes or targeting peptides, known to target different subcellular organelles, fused to a C. elegans optimized mOrange2 fluorescent protein (CemOrange2). These expression constructs were then injected into wild-type C. elegans to generate transgenic lines for studying protein trafficking, colocalization studies and complex biological questions in the context of a whole organism.

Publication: CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans


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Poranki, Deepika


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