Plasmids were generated with the NHX-2 promoter, to restrict gene expression to the C. elegans intestinal cells, driving C. elegans genes or targeting peptides, known to target different subcellular organelles, fused to a C. elegans optimized mOrange2 fluorescent protein (CemOrange2). These expression constructs were then injected into wild-type C. elegans to generate transgenic lines for studying protein trafficking, colocalization studies and complex biological questions in the context of a whole organism.

Publication: CemOrange2 fusions facilitate multifluorophore subcellular imaging in C. elegans