— This mouse strain has a genetic alteration in STAT3 and can be used to understand the role of STAT3 in human disease. Publication: Early-onset lymphoproliferation and autoimmunity caused by germline STAT3 gain-of-function mutations
— This capsid expresses a dmoninant negative non-functional form of the SK2 channel present in neurons. This allows us to directly measure the effects of SK2 in hippocampal neurons.
— Murine tumor cell lines Murine syngeneic MCA induced tumor lines have been developed and characterized by the Schreiber Lab and provide critical models to study drugs or pathways that impact tumor growth or rejection. In particular they are used to study mechanism of action of immune checkpoint blo…
— This expression plasmid can be used to identify inhibitors for Keap1 – a pathway that is invoIved in oxidative stress response and implicated in Marburg virus infection. Publication: Dimerization controls Marburg virus VP24-dependent modulation of host antioxidative stress responses
— Gadd7 haploinsufficient mutant CHO cell line Inventors generated a mutant Chinese Hamster Ovary (CHO) cell line with disruption of one allele encoding Gadd7, a non-coding RNA. CHO cells were transduced with retrovirus at a low multiplicity of infection and mutants were isolated. Number of retrovira…
— Target: CD155 is a adhesion receptor, orginally known as the cellular receptor for Polio Virus. Notes: Clone name is SK II 4.3Information on antibody against poliovirus receptor (CD155) Herein, we describe the generation of a murine antibody that recognizes poliovirus receptor (CD155). We made this antibody by immunizing mice with SK-N-S1 human neuroblastoma cells. We then validated this antibody using flow cytometry. …
— Valosin Containing Protein (VCP) patient derived fibroblasts, reprogrammed into iPSC cells and corresponding gene corrected line. These cells can be used to model VCP associated muscle disease, ALS, and dementia.
— Target: Mouse/rat interleukin-1 beta and mouse interleukin-1 alpha. Located on macrophages, dendritic cells, T and B cells. Notes: Clone name is B-122 and Alf-161Information on antibodies against IL-1 beta: The role of murine IL-1 beta in vitro and in vivo has not been defined. We describe here the production of neutralizing and immunoprecipitating mAb and polyclonal antibodies specific for murine IL-1 beta and their application to a characterization of the…
— Target: Full length mouse Vb 8.3 TCR but not 8.1 or 8.2. Vb 8.3. Application: Flow cytometryThis antibody was made by immunizing Armenian hamsters. It was used to study the mechanisms that act to keep only one functional TCR beta-chain per cell and the effect of selecting ligand density on positive selection and endogenous receptor usage in mice. Publication: TCR transgenic mice in which …
— Target: CD96 is a T cell-specific receptor indicative of T cell activation. Notes: Clone name NK92.39.1. For use in flow cytometry.Researchers generated the antibodies by immunizing mice with NK92 cells and selected hybridomas that plocked binding of PVR to NK92. These antibodies have been used to study natural killer cell target cell adhesion. Publication: Cutting Edge: CD96 (Tactile) Promotes NK Cell-Target Cell Adhesion by …
— Crry-knockout mouse strain (Crry -/-)Researchers generated mice deficient in Crry by disrupting exon 5 of the mouse Crry gene. The mice are sensitive to complement deposition and activation, especially the C3 component of complement on the surface of the placenta. Publication: A Critical Role for Murine Complement Regulator Crry in Fe…
— Plasmid vectors for shRNA expression, CRISPR reagent delivery, protein expression, reporter (promoter or 3’UTR) assays, and non-coding RNA delivery Efficient gene delivery is problematic, especially in hard to transfect primary cells (e.g. organoids). Commercial piggybac vectors are sparse and …
— Ifnl2/Ifnl3 double knock-out mouse strain This mouse strain is in the C57BL/6 background with Ifnl2, Ifnl3 genes disrupted to create a double knock-out. It was generated with assistance from the Washington University GEIC. This strain was used to study the regulation of the innate immune response t…
— Technology Description Engineers in Prof. Vijay Ramani’s laboratory have developed an electrode decoupled redox flow battery (RFB) with stable electrolyte materials that can generate power densities comparable to all-Vanadium RFBs at less than half the cost. RFBs are a promising option for …
— Target: NKp44 is a novel triggering surface molecular specifcially expressed by activated natural killer cells. Notes: Clone name 448.189 Marco Colonna, MDInventors generated NKp44 monocolonal antibodies by immunizing mice with a NKp44-human crystallizable fragment (Fc) fusion protein. The antibodies have been used to study innate and adaptive immune responses. Publication: Paradoxic inhibition of human natural interferon-producing cells by the activ…
— Mouse strain deficient in IL-1 betaInventors created the IL-1 beta deficient mouse strain by targeting the IL-1 beta gene in embryonic stem cells. These mice develop and grow normally. Endotoxin-stimulated peritoneal macrophages from IL-1beta- deficient mice showed normal synthesis and cellular release of IL- 1alpha after treatment w…
— Technology Description Engineers in Prof. Roger Chamberlain’s laboratory have developed a low-cost multiplexing technology for high-speed optical communications in data centers and other short-haul applications. This visible light communication (VLC) system is designed to create distinct par…
— Background: Its unique imaging characteristics and favorable half-life and decay properties make radioisotope copper-64 (Cu-64) most desirable for PET imaging. To meet the increasing demand, an efficient high yielding Cu-64 manufacturing process is essential. To address this unmet need, scientists f…
— Researchers in Prof. Fuzhong Zhang’s laboratory have developed a simple, scalable, broadly-applicable system to enhance fermentation efficiency for biosynthetic production of biofuels, pharmaceuticals, materials and other useful chemicals. Currently, biosynthetic pathways have enabled renewabl…
— Background: Dementia caused by Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative diseases manifests as a progressive decline in memory and other cognitive abilities. Deposits of beta-amyloid plaques and tangles of the tau protein are thought to cause the destruction o…
