This plasmid was created to test the regulatory impact of 5’ untranslated regions in yeast. It consists of EGFP under the control of the S. cerevisiae TEF2 promoter with cloning sites to modify the 5’UTR.
Publication: Roy, B., Granas, D., Bragg, F., Cher, J. A., White, M. A., & Stormo, G. D. (2020). Autoregulation of Many Yeast Ribosomal Proteins Discovered by Efficient Search for Feedback Regulation. bioRxiv.