Summaries by Inventor Sibley, Laurence

Plasmids for expression of the enzyme PheRS
Sibley, Laurence

— Plasmids for expression of phenyl alanine tRNA synthase from Toxoplasma gondii, Plasmodium falciparum and Homo sapiens. These include wild type and various mutant forms.

Toxoplasma strains
Sibley, Laurence

— The inventors have generated multiple gene knockout strains of T. gondii, listed below. RH∆hx∆ku80 TIR1 (RH∆ku80∆hxgprt/pTub 5’UTR-OsTir1-3xFlag-DHFR 3’UTR-CAT): The inventors used the CRISPR/Cas9 system for genome editing, comined with the AID degradation system to generate th…

Antibodies against Toxoplasma gondii proteins ROP1, ROP5, GRA7
Sibley, Laurence

— Polyclonal or monoclonal antibodies against Toxoplasma gondii proteins (ROP1, ROP5, GRA7) Mouse anti-body ROP1 publication: Localization of a Toxoplasma gondii Rhoptry Protein by Immunoelectron Microscopy During and After Host Cell Penetration Polyclonal rabbit antibody ROP5 publication: Virulence…

Hybridoma Cell Lines SAG1 and MIC2
Sibley, Laurence

— 2 murine hybridoma cell lines producing antibodies agains Toxoplasma gondii proteins SAG1 and MIC2 SAG1 (DG52) publication: Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii MIC2 (6D10) publication: The Toxoplasma Adhesive Protein MIC2 Is Proteolytically Proces…

HFF Cell Line
Sibley, Laurence

— This human cell line is a generic source for culturing Toxoplasma gondii in the laboratory. It was generated by Michael White’s laboratory.

Plasmids for Cryptosporidium parvum
Funkhouser-Jones, Lisa ; Shen, Bang ; Sibley, Laurence

— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …

Plasmids for luciferase expression in Cryptosporidium
Funkhouser-Jones, Lisa ; Sibley, Laurence

— Plasmids for luciferase expression in Cryptosporidium The plasmids listed below are for targeting insertion into the TK and UPRT loci in Cryptosporidium parvum. To generate the plasmids, the inventors modified a previously described nanoluciferase (Nluc) reporter fused to neomycin resistance (NeoR)…


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