Summaries by Inventor Moon, Tae Seok

pAH198 — plasmid containing ColE1
Hoynes-O'Connor, Allison ; Moon, Tae Seok

— A plasmid containing the E. colil ColE1 origin of replication, a chloramphenicol (antibiotic) resistance cassette, and a tetracycline-inducible antisense RNA gene regulator containing a target binding region (TBR) and the synthetic MicF M7.4 Hfq binding site for additional stability in the RNA molec…

pDD82, pDD112 and pDD120 plasmids
Moon, Tae Seok

— Description: Plasmids- Publication: DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS. Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630. ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 10.1021/acssynbio.7b00416. Epub 2018 Feb 5.

pBAD.dCas9sth1.pAL5000 plasmid
Moon, Tae Seok

— A plasmid constuct containing the Streptococcus thermophilus dCas9 (dCas9sth 1) gene under the control of the arabinose inducible pBAD promoter and a constitutively expressed single guide RNA (sgRNA) on the pAL5000 vector backbone. Publication: Molecular Toolkit for Gene Expression Control and Geno…

pTet-T – A plasmid for chromosomal integration of pTet-fbfp & kanamycin antibiotic resistance into Synechocystis sp. PCC6803
Moon, Tae Seok

— The plasmid was constructed in E. coli DH10B which was grown in LB at 37°C and 250 rpm in 14 mL BD FalconTM round‐bottom tubes. Kanamycin (kan) (20 µg/mL) and spectinomycin (spec) (100 µg/mL) were added as appropriate. Plasmids were constructed using the…

Plasmids for pDD56, pDD57, pDD87, pDD103, pDD108, pDD112, pDD120, pDD131, pDD132, and pDD143
Moon, Tae Seok

— o pDD56: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance. o pDD57: a shuttle vector between Escherichia coli and Rhodococcus opacus PD630 that contains a constitutively expressed GFP+ on…

DNA Plasmids for the Modulation of Gene Expression
Lee, Young Je (Ryan) ; Moon, Tae Seok

— These set of plasmids allow for the repression and de-repression of fluorescent proteins (GFP and mCherry) through CRISPR interference and antisense RNA. Publication: Programmable control of bacterial gene expression with the combined CRISPR and antisense RNA system

DNA plasmids to control gene expression in E. coli
Hoynes-O'Connor, Allison ; Moon, Tae Seok

— These plasmids contain target binding regions (TBRs) targeting rfp that were placed under the control of pTet via blunt end lifgation. Hfq binding sites that had been identified from the literature were inserted adjacent to the TBR sequence using either blunt end ligation or Golden Gate assembly, de…


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