Summaries by Inventor Funkhouser-Jones, Lisa

Plasmids for Cryptosporidium parvum
Funkhouser-Jones, Lisa ; Shen, Bang ; Sibley, Laurence

— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …

Plasmids for luciferase expression in Cryptosporidium
Funkhouser-Jones, Lisa ; Sibley, Laurence

— Plasmids for luciferase expression in Cryptosporidium The plasmids listed below are for targeting insertion into the TK and UPRT loci in Cryptosporidium parvum. To generate the plasmids, the inventors modified a previously described nanoluciferase (Nluc) reporter fused to neomycin resistance (NeoR)…


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