Antibodies against Toxoplasma gondii proteins ROP1, ROP5, GRA7

— Polyclonal or monoclonal antibodies against Toxoplasma gondii proteins (ROP1, ROP5, GRA7)

Mouse anti-body ROP1 publication: Localization of a Toxoplasma gondii Rhoptry Protein by Immunoelectron Microscopy During and After Host Cell Penetration

Polyclonal rabbit antibody ROP5 publication: Virulence…

Toxoplasma strains

— The inventors have generated multiple gene knockout strains of T. gondii, listed below.

RH∆hx∆ku80 TIR1 (RH∆ku80∆hxgprt/pTub 5’UTR-OsTir1-3xFlag-DHFR 3’UTR-CAT):

The inventors used the CRISPR/Cas9 system for genome editing, comined with the AID degradation system to generate th…

Hybridoma Cell Lines SAG1 and MIC2

— 2 murine hybridoma cell lines producing antibodies agains Toxoplasma gondii proteins SAG1 and MIC2

SAG1 (DG52) publication: Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii

MIC2 (6D10) publication: The Toxoplasma Adhesive Protein MIC2 Is Proteolytically Proces…

HFF Cell Line

— This human cell line is a generic source for culturing Toxoplasma gondii in the laboratory. It was generated by Michael White's laboratory.

Plasmids for Cryptosporidium parvum

— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum

To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …

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